Use of the MILLIPLEX® bovine cytokine/chemokine multiplex assay to identify Mycobacterium bovis-infection biomarkers in African buffaloes (Syncerus caffer)

Katrin Smith,Léanie Kleynhans,Candice Snyders,Netanya Bernitz,David Cooper,Paul van Helden,Robin M. Warren,Michele A. Miller,Wynand J. Goosen

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY（2021）

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摘要

•MILLIPLEX® bovine multiplex assay detected African buffalo cytokines/chemokines.•Twelve of 15 cytokine targets showed significant mitogen-induced responses.•Seven cytokines were increased in TB antigen-samples from infected buffaloes.•Promising tool for screening African buffalo cell-mediated immune responses.

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关键词

African buffalo, Bovine cytokine/chemokine, Luminex (R), Mycobacterium bovis, MILLIPLEX (R) multiplex assay, QuantiFERON((R)) TB Gold plus

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