Biosynthesis of cycloartenol by expression of plant and bacterial oxidosqualene cyclases in engineered Rhodobacter capsulatus
Journal of Biotechnology: X(2019)
Abstract
•R. capsulatus is suitable for expression of plant/microbial cycloartenol synthases.•Titers were independent of chosen plant and myxobacterial cycloartenol synthases.•Co-expression of the mevalonate pathway had little impact on cycloartenol levels.•Deletion of crtE led to threefold increased cycloartenol product titers.
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Key words
MEP,MVA,G3P,IPP,DMAPP,GPP,FPP,GGPP,Idi,CrtE,SQS,SQE,OSC,CAS,CBC,At-CAS,SaSg-CAS,SaDW-CAS
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