EFFECT OF EXTRACTION SOLVENTS IN BIOLOGICAL ACTIVITY OF EXTRACTS OF POTATO BYPRODUCTS (Solanum tuberosum)

In this study the effect of different solvents for extraction of bioactive substances from peel and sprout of the potato variety Fianna were evaluated. To define the best extraction with solvents such as water (Ac), ammonium hydroxide (HA) and ethanol-acid (EA), a phytochemical study was conducted and an antimicrobial evaluation with the bacteria E. coli O157:H7, S. typhimurium, S. aureus and L. ivanovii. A variability was detected in peel and sprout extracts between solvent of extraction. The phytochemical analysis showed the presence of several groups of compounds among which included alkaloids, steroids and carbohydrates. The antimicrobial evaluation showed that the Ac extractions exhibited no antibacterial activity and HA and EA extractions were more effective in eliminating S. aureus and L. ivanovii. E. coli O157: H7. In contrast S. typhimurium showed increased resistance to these extracts. For the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) analysis, EA extract was used due to its higher antimicrobial activity. The MIC was between 6.25 to 50 mg/mL and 25 MBC and > 50 mg/mL. These results allows to consider the potential use of these extracts.