The histological and physiological potential of obestatin on testicular ischemia-reperfusion induced injury in adult albino rats

Testicular ischemia reperfusion (IR) is a critical trouble in males in different ages. This trouble takes place due to the torsion and detorsion of testis. It has been manifested that obestatin have anti- inflammatory and protective effects on ischemia reperfusion injury in the kidney, brain and heart. However, its actions on testicular ischemia reperfusion injury have not been evaluated. We seek to examine the actions of obestatin on rat testicular ischemia/ reperfusion induced by torsion/ detorsion and the potential following mechanisms. In this study, we divided 40 adult male albino rats weighing (150- 180) gm equally into 4 groups as follow: (I) control "sham" group"; (II) testicular ischemia reperfusion (IR) group, 2 hours of ischemia followed by 2 hours of reperfusion, (III) ischemia reperfusion group treated by saline (0.5 m) administered after ischemia reperfusion and (IV) ischemia reperfusion group treated by obestatin (8 nmol/Kg/body weight). After doing surgery to all rats, some testicular oxidative stress markers and serum free testosterone were measured and histopathologic study. Significant decrease in serum testosterone levels, glutathione peroxidase (Gpx) activity and testicular superoxide dismutase (SOD) and increase in tissue malondialdehyde (MDA) and increase in testicular weight were observed in ischemia reperfusion group comparing with the control group. These antioxidant levels were increased in obestatin treated groups with significant decrease in MDA levels. Also, obestatin increase serum free testosterone. These results manifested that obestatin guards against the damage caused by ischemia reperfusion on testicular structure and function when used for 7 days by dose of 8 nmol/kg/body weight its anti -oxidant mechanism.