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Rapid multiplex PCR assay for simultaneous detection of Neisseria gonorrhoeae and Chlamydia trachomatis in genitourinary samples: A 30-minute assay

Takeshi Inoue,Yoshitomo Chihara,Keisuke Kiba,Shuya Hirao,Masahiro Tanaka,Tatsuo Yoneda,Toshihisa Saka, Keiichi Furubayashi, Ken-Ichi Beppu, Mizuki Takashima,Hidenori Nagai,Hiroshi Kobayashi,Kiyohide Fujimoto

Journal of Microbiological Methods（2021）

Cited 5|Views18
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Abstract
We developed a rapid multiplex PCR assay available in bedside for the simultaneous detection of Neisseria gonorrhoeae and Chlamydia trachomatis, which enables diagnosis in less than 30 minutes. In this study, we validated the clinical utility of this assay including its sensitivity and specificity for NG and CT detection.
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Key words
Sexually transmitted infections,N. gonorrhoeae,C. trachomatis,Rapid multiplex PCR,Nucleic acid amplification tests
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