Dynamic Distribution of Gut Microbiota in Chickens with Embryonic Development.

ABSTRACT The gut microbiota is a complex ecological community and widely recognized in many aspects of research, but little is known on the relation between gut microbiota and embryonic development in chicken. The aim of this study was to explore the dynamic distribution of gut microbiota in chickens’ embryo during stages of developments (chicken embryos that had incubated to day 3 (E3), day 12 (E12) and day 19 (E19)). Here, 16S rRNA gene sequencing was performed on the gut microbiota in chickens’ embryo across different developmental stages. 21 phyla and 601 genera were present in the chicken embryos and 96 genera such as Ochrobactrum, Phylobacterium, and Amycolatopsis were core microbiota in the three stages of development. Secondly, 94 genera of microbes were observed to change significantly between E3 and E12, 143 genera significantly different between E12 and E19 of chicken embryo (P