The Treatment Effect of Highly Biocompatible Bismuth/ strontium/hydroxyapatite/chitosan for Osteosarcoma, Bacterial and Bone Defects

Bi particles are dispersed in hydroxyapatite/ strontium/chitosan material by mixed coprecipitation and freeze-drying method. In oder to assess their anti-tumor, osteogenic and antibacterial effects, SEM, EDS, FIRT, XRD, hemolysis and swell experiments were used to assess the physical properties of the material. Cytotoxicity assays, PCR techniques were used to assess the effects of material on bone cells, osteosarcoma cells. Flow cytometry was used to evaluate the effect of composites on osteosarcoma cells, and the inhibitory effect of material on Staphylococcus aureus was evaluated by antimicrobial susceptibility test. The results show that Bi/Sr/CS/HA composites have better anti-tumor, anti-infective and osteogenic functions than Sr/CS/HA composites with increasing niobium concentration. Furthermore, by analyzing the results, it is believed that the addition of Bi in composite material play important roles in enhancing the antibacterial, antitumor and osteogenesis of the composite.