Employing Predicted Response Factors And A Validated Chromatographic Method For The !Relative Quantitation Of Holy Basii Essential Oils
JOURNAL OF ESSENTIAL OIL RESEARCH(2020)
Abstract
This work reports on fourteen holy basil (Ocimum tenuiflorum L., O. gratissimum L.) essential oil profiles, and a reliable method for quantitation of essential oil constituents via a combination of external and internal standardization approaches using predicted relative response factors (RRFs). Results demonstrate one variety was composed primarily of methyl eugenol (similar to 65 %), and another of eugenol (67%). Multiple varieties contained moderate eugenol (similar to 37-45%) and beta-caryophyllene (similar to 23-37%) levels, while others possessed equally fair quantities of eugenol, eucalyptol, estragole, and beta-bisabolene. The developed method showed good precision and repeatability with a relative standard deviation (RSD) < 4% for all tests. The reliability of predicted RRFs was evaluated with a difference of 0.96 +/- 0.25 to 11.21 +/- 1.34% between the two quantitation methods. High concentration compounds showed greater differences between calculated and predicted values. Finally, an HP-5 column provided excellent resolution, except for essential oils containing methyl eugenol and beta-caryophyllene.
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Key words
Holy basil, Ocimum tenuiflorum, Ocimum gratissimum, essential oils, gas chromatography, predicted relative response factors
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