Employing Predicted Response Factors And A Validated Chromatographic Method For The !Relative Quantitation Of Holy Basii Essential Oils

This work reports on fourteen holy basil (Ocimum tenuiflorum L., O. gratissimum L.) essential oil profiles, and a reliable method for quantitation of essential oil constituents via a combination of external and internal standardization approaches using predicted relative response factors (RRFs). Results demonstrate one variety was composed primarily of methyl eugenol (similar to 65 %), and another of eugenol (67%). Multiple varieties contained moderate eugenol (similar to 37-45%) and beta-caryophyllene (similar to 23-37%) levels, while others possessed equally fair quantities of eugenol, eucalyptol, estragole, and beta-bisabolene. The developed method showed good precision and repeatability with a relative standard deviation (RSD) < 4% for all tests. The reliability of predicted RRFs was evaluated with a difference of 0.96 +/- 0.25 to 11.21 +/- 1.34% between the two quantitation methods. High concentration compounds showed greater differences between calculated and predicted values. Finally, an HP-5 column provided excellent resolution, except for essential oils containing methyl eugenol and beta-caryophyllene.