感染赤星病烟草叶际细菌的多样性分析
Tobacco Science & Technology(2020)
Abstract
为揭示田间感染赤星病烟草叶际细菌属水平上的多样性,提取了感病烟叶样品DNA,PCR扩增样品的16S区,采用IonS5XL高通量测序技术对扩增片段测序,并对其细菌群落结构进行分析.结果表明,田间感赤星病烟草叶片叶际细菌群落结构较为典型.在门水平上,优势菌门为变形菌门Proteobacteria(71.59%);属水平上,优势菌为泛菌属Pantoea(31.23%)、假单胞菌属Pseudomonas(22.44%)、甲醇杆菌属Methylobacterium(6.17%)、鞘氨醇单胞菌属Sphingomonas(6.02%)、Aureimonas(2.08%)、Falsirhodobacter(1.35%).因此,感烟草赤星病叶片叶际优势细菌属为泛菌属和假单胞菌属.
MoreKey words
bacterial genus,tobacco leaves
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