FK506促进乳鼠雪旺细胞增殖的实验研究
Chinese Journal of Hand Surgery（2005）
Abstract
目的观察FK506对体外培养的乳鼠雪旺细胞增殖的影响.方法将纯化的雪旺细胞分两组,一组设为对照,另一种加含终浓度为0.5ng/ml FK506的DMEM/F12培养液培养,用MTT法检测不同时间点的OD值并绘制生长曲线,另在培养第48、72小时后用3H-胸腺嘧啶核甙测定法检测其DPM值.结果经含0.5ng/mlFK506培养液培养的雪旺细胞,其对数生长期提前出现,并且在峰值上高于对照组;接种后48h和72h,3H-胸腺嘧啶核甙检测DPM值实验组也高于对照组,差异有统计学意义(P<0.05).结论 FK506有促进雪旺细胞分裂增殖的作用,可能是其促进周围神经再生的重要机制之一.
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Key words
schwann cells,vitro,proliferation,fk506
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