HPLC法对多厂家小儿感冒颗粒中连翘酯苷A等5种成分的含量分析
Zhong yi xue(2019)
Abstract
目的 建立同时测定小儿感冒颗粒中连翘酯苷A、连翘苷、(R,S)-告依春、绿原酸和异绿原酸A含量的HPLC法,并对市售16批样品进行含量测定.方法 采用HPLC法,Kinetex EVO C18色谱柱(150 mm×4.6 mm, 5 μm),流动相为乙腈-0.3%磷酸溶液,梯度洗脱;连翘苷和(R,S)-告依春的检测波长为236 nm,连翘酯苷A、绿原酸和异绿原酸A的检测波长为327 nm;流速1.0 ml/min;柱温35 ℃.结果 连翘酯苷A、连翘苷、(R,S)-告依春、绿原酸和异绿原酸A分别在0.050 45~2.018 00 μg (r=0.999 9)、0.018 21~0.728 40 μg(r=0.999 9)、0.010 16~0.406 40 μg(r=0.999 9)、0.006 60~0.263 90 μg (r=0.999 9)、0.004 04~0.161 76 μg(r=0.999 5)范围内呈良好的线性关系;重复性、稳定性的RSD均小于2%;加样回收率分别为97.01%、98.28%、99.35%、96.21%和98.75%,RSD为3.19%、1.19%、0.81%、2.88%和2.96%.14家生产厂家的16批样品中连翘酯苷A、连翘苷、(R,S)-告依春、绿原酸和异绿原酸A的含量范围分别为0.057 43~1.508 71 mg/g、0.017 72~0.350 15 mg/g、0.005 68~0.177 13 mg/g、0.007 53~0.226 33 mg/g和0.003 08~0.119 08 mg/g.结论 该方法操作简便、结果准确、稳定性良好,适用于测定小儿感冒颗粒中连翘酯苷A、连翘苷、(R,S)-告依春、绿原酸和异绿原酸A含量;市售样品中,连翘酯苷A等5种成分含量相差较大,应加强质量控制.
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Key words
Xiao Er Gan Mao Ke Li,Forsythoside A,Phillyrin,(R,S)-Epigoitrin,Chlorgenic Acid,Isochlorogenic Acid A,HPLC
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