Study of HBV genotypes andYMDD mutations by reverse dot blot
Chinese Journal of Laboratory Medicine(2006)
Abstract
目的 应用逆向斑点杂交技术检测乙型肝炎病毒(HBV)基因型及YMDD变异.方法合成有生物素标记的引物,PCR扩增两种DNA片段,与尼龙膜上的探针同时杂交,BCIP/NBT显色.结果检测21例拉米夫啶治疗中的慢性乙型肝炎患者,11例HBV为基因型B,10例为基因型C;YMDD基序有47.6%(10/21)为YMDD,28.6%(6/21)为YVDD,23.8%(5/21)为YIDD.结论在同一张尼龙膜上检测HBV基因型及YMDD变异,经济实惠、准确快捷,且易于开展。
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Key words
hbv genotypes andymdd mutations
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