Analysis of virulence genes and drug resistance in 82 Vibrio parahaemolyticus strains

Objective\r\nTo analyze the virulence genes and drug resistance in Vibrio parahaemolyticus strains isolated in Nantong City from 2015 to 2016 in order to provide reference for the prevention and treatment of Vibrio parahaemolyticus infection and for rational use of medicines.\r\n\r\n\r\nMethods\r\nVirulence genes of tlh, tdh and trh in Vibrio parahaemolyticus strains were detected by fluorescence quantitative PCR. Micro-broth dilution method was used to analyze antimicrobial resistance in these strains to 15 kinds of antibiotics.\r\n\r\n\r\nResults\r\nEighty-two Vibrio parahaemolyticus strains were all positive for tlh gene and negative for trh gene and among them, 72 carried tdh gene (87.8%). Antimicrobial resistance rates of these strains to ampicillin, cefazolin, tetracycline and chloramphenicol were all 1.2% (1/82). Two strains (2.4%) were resistant to trimethoprim/sulfamethoxazole. All strains were sensitive or intermediate to another 10 kinds of antibiotics.\r\n\r\n\r\nConclusion\r\nFrom 2015 to 2016, Vibrio parahaemolyticus strains carrying virulence genes of tlh and tdh were prevalent in Nantong and no trh gene-positive strains were reported. Except ampicillin, cefazolin, tetracycline, chloramphenicol and trimethoprim/sulfamethoxazole these five kinds of antibiotics, the remaining 10 kinds of antibiotics were effective against Vibrio parahaemolyticus and could be used as the treatment of choice.\r\n\r\n\r\nKey words: \r\nVibrio parahaemolyticus; Virulence gene; Drug susceptibility test