Isolation, identification and antimicrobial activity of secondary metabolites of endophytic fungi from annona leaves (Annona squamosa L.) growing in dry land

Research on isolation, identification, and antimicrobial activity of secondary metabolites of endophytic fungi from leaves of Annona squamosa growing in dryland had been undertaken. The work includes the isolation of endophytic fungi, cultivation, and extraction of fungal extract and identification of chemical metabolites together with the antibacterial test. The pure colony of endophytic fungi was grown on solid rice media using 1 L Erlenmeyer flask. The grown fungi were extracted with ethyl acetate. The ethyl acetate crude extract was then further subjected to chemical analysis and its antibacterial properties. Endophytic fungi species was identified as Aspergillus niger based on macroscopic and microscopic analysis. Analysis LC-MS/MS had revealed the presence of five metabolites including ephedradine A, ergosine, Ia, mudanpioside H, and trichosanic acid. The extract showed strong inhibition against Staphyloccus aureus with diameter of zone inhibition of 16.1 mm and moderate inhibition against Escherichia coli 0175H7 and Salmonella enteritidis ATCC 6939 with the observed diameter of zone inhibition of 9.6 mm and 11.3 mm, respectively.