UNIT 10C.1 Laboratory Maintenance of Mycobacterium smegmatis

This unit gives background information on Mycobacterium smegmatis, a mycobacterial model system, and covers all the laboratory maintenance for this species including growth in liquid and on solid medium. It also contains recommendations concerning long-term strain storage. Although M. smegmatis is a Biosafety Level 1 organism, some rare infections in humans have been reported, and, thus all of the required safety measures are discussed here. Curr. Protoc. Microbiol. 14:10C.1.1-10C.1.12. © 2009 by John Wiley \u0026 Sons, Inc.\r\n\r\n\r\nKeywords:\r\n\r\nmedium;\r\nMiddlebrook;\r\n7H9;\r\nSauton;\r\nLB Miller;\r\nstrain storage