Tu1299 HELICOBACTER_BILIS MAY PLAY A ROLE IN THE CARCINOGENESIS OF COLITIS ASSOCIATED COLON CANCER CORRELATING TO INCREASED NUMBER OF CD4+CD45RB+ T CELLS

Background The role of Helicobacter bilis (H.bilis) in the development of inflammatory bowel disease (IBD) and colitis-associated carcinogenesis (CAC) has seldom been investigated. Methods Using 16S rRNA fluorescence in situ hybridization (FISH), we examined the abundance of H.bilis in 58 colorectal cancers (CRCs), 20 IBDs, 40 normal colorectal mucosae (NCs) and 20 adenomas (ADs). Number of CD4+CD45RB+T cell and expression of IFN-γ and TNF-α in these tissues was determined by immunofluorescence. Results The abundance of H.bilis was significantly higher in CRCs (33.7±27.7) than that in IBDs (15.0±16.3; P = 0.006), ADs (4.01±7.3; P Conclusions H.bilis may play a role in the initiation of IBD and CAC, possibly through promoting the transformation of T cells into CD4+CD45RB+T cells and increasing the expression of proinflammatory cytokines IFN-γ. Legal entity responsible for the study Xiangsheng Fu. Funding Has not received any funding. Disclosure All authors have declared no conflicts of interest.