Development of Loop Mediated Isothermal Amplification (LAMP) Assay for Detection of PCV2

Porcine circovirus2 (PCV2) is an emerging pathogen of porcine farming. PCV2 is a non-enveloped circular single-stranded DNA virus. PCV2 infection produces variety clinical symptoms and thus causes huge economic loss. Various diagnostic methods are available for the diagnosis of PCV2 infection. However, there is still no rapid, sensitive and practical method for detecting PCV2. Loop mediated isothermal amplification (LAMP) has been used to detect a variety of pathogenic microorganisms. In this study, we developed a LAMP for detection of PCV2. Primers used for LAMP were designed using conserved region of PCV2 sequences available in Genbank. The LAMP assay was developed by standard protocol including determination sensitivity and specificity of the assay. The LAMP assay provides a rapid, sensitive, reliable way to detect PCV2 and can be used for the epidemiological investigation of PCV2.