Osteogenic growth peptide-loaded 3D-printed PCL scaffolds for the promotion of osteogenesis through the ERK pathway
Materials & Design(2020)
Abstract
This study aims to improve the osteogenic differentiation in vitro and bone formation in vivo through polycaprolactone (PCL) scaffolds modified with the osteogenic growth peptide (OGP). Three-dimensional (3D)-printed PCL scaffolds were designed with computer-aided design (CAD) software and fabricated by 3D layer-by-layer fused deposition. After the scaffold surfaces were modified by surface amination, the OGP was successfully loaded onto the scaffolds using a electrostatic self-assembly method. The effects of these scaffolds on the biocompatibility and osteogenic differentiation of rat bone marrow-derived stem cells (BMSCs) were studied in vitro. The modification did not influence the adhesion or proliferation, but had a notable osteogenic effect in vitro. This may be attributed to the activation of the mitogen-activated protein kinase/extracellular regulated protein kinase (MAPK/ERK) signalling pathway. Based on the results obtained after implantation into a rat cranial defect model, the PCL/OGP scaffolds notably promoted bone formation and accelerated mineralisation. The innovative modification method introduced in this work, will improve the application of PCL scaffolds in tissue engineering.
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Key words
Polycaprolactone,1,6-hexamethylenediamine,Bone marrow-derived stem cells,Osteogenic growth peptide,Osteogenic differentiation,Cranial defect model
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