NEAT1 aggravates sepsis-induced acute kidney injury by sponging miR-22-3p
OPEN MEDICINE(2020)
Abstract
Background and aim - Acute kidney injury (AKI) is a common complication of sepsis. Long noncoding RNA nuclear-enriched abundant transcript 1 (NEAT1) plays a vital role in various diseases, including AKI. This study aimed to investigate the function and mechanism of NEAT1 in sepsis-induced AKI. Materials and methods - A septic AKI model was established by treating HK-2 cells with lipopolysaccharide (LPS). The levels of NEAT1 and miR-22-3p were measured by quantitative real-time PCR. Cell apoptosis was assessed by flow cytometry. The levels of apoptosis-related protein and autophagy-related factors were examined by the western blot assay. An enzyme-linked immunosorbent assay was used to calculate the contents of inflammatory factors. The interaction between NEAT1 and miR-22-3p was validated by dual-luciferase reporter assay, RNA immunoprecipitation assay, and RNA pull-down assay. The levels of nuclear factor (NF)-kappa B pathway-related proteins were evaluated by the western blot assay. Results - NEAT1 was upregulated, while miR-22-3p was downregulated in patients with sepsis and in LPS-stimulated HK-2 cells. LPS treatment triggered cell apoptosis, autophagy, and inflammatory response in HK-2 cells. NEAT1 knockdown attenuated LPS-induced cell injury. NEAT1 modulated LPS-triggered cell injury by targeting miR-22-3p. Furthermore, NEAT1 regulated the NF-kappa B pathway by modulating miR-22-3p. Conclusion - Depletion of NEAT1 alleviated sepsis-induced AKI via regulating the miR-22-3p/NF-kappa B pathway.
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Key words
sepsis,NEAT1,miR-22-3p,acute kidney injury,NF-kappa B pathway
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