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Enhanced chitin gel with magnetic nanofiller for lysozyme purification

Gabriel Ibrahin Tovar,Roberto Fernández de Luis,María Isabel Arriortua,Federico Javier Wolman,Guillermo Javier Copello

Journal of Industrial and Engineering Chemistry（2020）

Cited 3|Views11
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Abstract
•Nanofillers of Fe3O4 were embedded in chitin for lysozyme purification.•The increase of Fe3O4 modified the cell parameters and porosity of chitin.•Nanofillers influenced chitin structure enhancing lysozyme purification.•This nanocomposite allows one step purification of hen egg white lysozyme.•A 75% global yield and 99% lysozyme purity was obtained from undiluted egg white.
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Key words
Chitin,Magnetite,Nanofiller,Protein purification,Lysozyme
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