Antioxidant Effect of a Vegetal Grape Waste Complex, Demonstrated in Relevant Dermal and Epidermal Cellular Systems

The complete capitalisation of vegetal raw materials as renewable natural resources is a current study direction that is based on the diversification of active compounds identified and isolated from certain sources, the effectiveness of extracting technologies and the definition of a relevant spectrum of biological action. The study is focused on highlighting the antioxidant effect induced by components of winemaking waste (TES extract) in dermo-epidermal cells. The tests was performed on normal fibroblast and normal human keratinocytic cell lines, preceded by acellular screening for DPPH free radical reduction and total antioxidant capacity assessment. At the cellular level, intracellular oxygenated oxygen (superoxide and hydrogen peroxide) and intracellular glutathione were monitored by flow cytometry, comparing with the intracellular antioxidant enzyme activity (Superoxide dismutase ant Catalase). The results confirm a concerted antioxidant effect for TES, suggesting many pharmaceutical and cosmetic applications.