Lab Resource: Stem Cell LineCharacterization of dermal fibroblast-derived iPSCs from a patient with high grade steatosis

Primary fibroblasts from a high grade steatosis patient were reprogrammed by transduction of retroviruses OCT4, SOX2, c-MYC and KLF4. IPSCs were characterized by immunocytochemistry, embryoid body-formation, DNA-fingerprint, karyotype analysis and comparative transcriptome analyses with the human embryonic stem cell line H1 revealed a Pearsons correlation coefficient of 0.9287.\r\n\r\nResource table.\r\n\r\n\tName of stem cell constructS08\t\r\n\tInstitutionInstitute for Stem Cell Research and Regenerative Medicine\t\r\n\tPerson who created resourceJustyna Jozefczuk\t\r\n\tContact person and emailJames Adjaye, James.Adjaye@med.uni-duesseldorf.de\t\r\n\tDate archived/stock dateAugust 2012\t\r\n\tOriginPrimary human fibroblasts\t\r\n\tType of resourceBiological reagent: induced pluripotent stem cell (iPSC); derived from steatosis patients and a healthy individual\t\r\n\tSub-typeCell line\t\r\n\tKey transcription factorsOCT4, SOX2, c-MYC and KLF4\t\r\n\tAuthenticationIdentity and purity of cell line confirmed (Figs. 1, 2)\t\r\n\tLink to related literature (direct URL links and full references)http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4672680/\t\r\n\tInformation in public databasesNone