P592 Microbiota concordance between mid-vaginal swabs and both clean- and random-catch urine samples
Sexually Transmitted Infections(2019)
摘要
Background While urine has successfully been used for STI testing, it has not been routinely used in urogenital microbiota studies. This work explores whether random-catch and/or clean-catch urine could be a proxy for assessing the vaginal microbiota. Methods In two studies, urinary and vaginal microbiota from women ages 17–45 were compared for (1) 91 participants with paired mid-vaginal swabs and random catch urine samples and (2) 99 participants with paired mid-vaginal swabs and clean catch urine samples. Microbiota composition was characterized by amplicon sequencing of the V3-V4 regions of the 16S rRNA gene. Taxonomic classification was assigned based on SILVA and SpeciateIt. Community State Types (CST) were assigned using an algorithm trained on 13,000 well-characterized samples. CST I, II, III, and V were dominated by: Lactobacillus crispatus, L. gasseri, L. iners, and L. jensenii, respectively. CST IV-A, IV-B, and IV-C represented low-Lactobacillus states. Similarity of paired urine and vaginal samples was measured at the CST-level by kappa statistics and the population-level with the Yue-Clayton theta indices. Results We obtained 12 and 7.8 million sequences from urine and vaginal samples, respectively. At the CST-level, random-catch and clean-catch urines were 82.4% and 81.8% concordant with paired mid-vaginal swabs, respectively. Substantial agreement was observed between urine and paired vaginal specimen (Κrandom-catch= 0.770 and Κclean-catch=0.743). At a population-level, average similarity of random- and clean-catch samples to paired vaginal samples indicated a high degree of similarity (θ=0.7496 and 0.7565, respectively). Comparison of the distributions of random-catch and clean-catch θ similarity scores showed no differences (p=0.86). Conclusion Bacterial compositions of random catch and clean catch urine samples showed substantial agreement to paired mid-vaginal samples assessed by CST- and community-level analyses. Random and clean catch urine samples could potentially be used as a proxy for vaginal microbiota in studies assessing the urogenital microbiota. Disclosure No significant relationships.
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