Distribution of Eleven Markers in South Romanian (walachia Region) Population

The South Romanian population has not been well studied at the genetic level. This study aims to investigate the genetic distribution of di-allelic DNA polymorphisms associated with different diseases in the South Romanian population. We investigated eleven di-allelic DNA polymorphisms in blood samples from 648 healthy Caucasian subjects (age 18-66 years old) from Bucharest and eight South Romanian districts. All markers present no significant deviation from expected Hardy-Weinberg equilibrium even if subjects were stratified according to the birth place, gender or age group. The frequency of allelic variants and genotypes in South Romanian areas situates in the range of values found with other Caucasian populations. When testing the distribution between the tested districts only VDR Fok was found to present a different distribution in samples from Bucharest and in those from Calarasi, Dambovita or Teleorman (p < 0.00008). Excepting MTHFR and MTR no other difference was detected between the age grouped subjects. MTHFR 677T allele increases progressively from subjects of 59-66 years old to those with 18-26 years (21.7% vs. 41%, p = 0.003). The highest difference of heterozygosity was found between the subjects in Bucharest and Giurgiu or Olt.