Ornithinimicrobium pratense sp. nov., isolated from meadow soil

A novel Gram-stain-positive, yellow, short-rod-shaped or coccoid bacterial strain, W204(T), was isolated from a soil sample collected from Jiadengyu national forest park in China and characterized using a polyphasic approach. The cell-wall peptidoglycan contained ornithine as the diagnostic diamino acid. 16S rRNA gene sequence analysis indicated that strain W204(T) was closely related to Ornithinimicrobium flavum CPCC 203535(T) (97.4%, similarity), Serinicoccus profundi CGMCC 4.5582(T) (96.9%), Serinicoccus sediminis GP-T3-3(T) (96.8%), Serinicoccus hydrothermalis JLT9(T) (96.7%), Ornithinimicrobium cerasi CPCC 203383(T) (96.6%) and Ornithinimicrobium kibberense K22-20(T) (96.6%). However, the digital DNA-DNA genome hybridization value between strain W204(T) and the closest related strain O. flavum CPCC 203535(T) was 21.90 %. Complete genome analyses revealed that the size of the genome was 3.54 Mb and the genomic DNA G+C content was 70.79 mol%. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, an unidentified glycolipid, an unidentified phospholipid and an unidentified lipid. The major menaquinone was MK-8(H-4). The predominant cellular fatty acids were iso-C-15:0, anteiso-C-15:0 and C-16:0. The phenotypic, chemotaxonomic and phylogenetic data suggested that strain W204(T) should be classified as representative of a novel species of the genus Ornithinimicrobium, for which the name Ornithinimicrobium pratense sp. nov. is proposed. The type strain is W204(T) (=GDMCC 1.1391(T)=KCTC 49237(T)).