Psychroflexus Aurantiacus Sp. Nov., Isolated From Soil In The Yellow River Delta Wetlands

A Gram-stain-negative, strictly aerobic, non-motile, orange-coloured bacterium, designated YR1-1(T), was isolated from a soil sample collected from the Yellow River Delta wetlands (PR China). Growth was observed at a salinity of 1.0-15.0% NaCl, 4-45 degrees C and pH 6.0-9.0. The results of phylogenetic analysis based on the 16S rRNA gene sequences indicated that YR1-1(T) represented a member of the genus Psychroflexus, with the highest sequence similarity to Psychroflexus sediminis YIM-C238(T) (97.9%), followed by Psychroflexus aestuariivivens (97.1%) and Psychroflexus torquis (96.4%). The average nucleotide identity and digital DNA-DNA hybridization values between YR1-1(T) and other closely related type strains of species of the genus Psychroflexus were 68.7-86.3% and 17.8-30.9%. The genome of the strain was 2 899374 bp in length with 39.8% DNA G+C content. The predominant fatty acids (>10 %) were iso-C-15:0 and anteiso-C-15:0. The major respiratory quinone was menaquinone-6 (MK-6) and the major polar lipids were phosphatidylethanolamine, phospholipid, diphosphatidylglycerol, two unidentified aminolipids and four unidentified lipids. The combined genotypic and phenotypic data indicate that YR1-1(T) represents a novel species within the genus Psychroflexus, for which the name Psychroflexus aurantiacus sp. nov., is proposed. The type strain is YR1-1(T) (=KCTC 72794(T)=CGMCC 1.17458(T)).