Salivary Biomarkers For Discriminating Periodontitis In The Presence Of Diabetes

Aim Salivary biomarkers can help in assessment of periodontitis; however, concentrations may be altered in the presence of diabetes. Hence, the ability of salivary biomarkers to discriminate periodontally healthy type II diabetics (T2DM) from T2DM who have periodontitis was examined.Methods Ninety-two participants (29 with T2DM with chronic periodontitis, DWP; 32 T2DM without chronic periodontitis, DWoP; and 31 Not Periodontitis, NP) provided saliva and clinical parameters of periodontal health were recorded. Salivary concentrations of interleukin (IL)-1 beta, IL-6, matrix metalloproteinase-8 (MMP-8), macrophage inflammatory protein-1 alpha (MIP-1 alpha), adiponectin and resistin were measured by immunoassay.Results Salivary analyte concentrations for IL-1 beta, MMP-8 and resistin correlated with clinical parameters of periodontitis, with MMP-8 demonstrating the strongest positive correlation with PD >= 5 mm (p < 0.0001). Periodontal health was reflected in salivary analyte concentrations by group, with concentrations of IL-1 beta and MMP-8 showing significant associations with periodontitis (p <= 0.04) that increased in concentration from health to DWoP to DWP. Odds ratio (OR) analyses showed that MMP-8 discriminated periodontitis from NP (OR of 8.12; 95% CI: 1.01-65.33; p = 0.03) and in the presence of T2DM (DWP vs DWoP, OR = 5.09; 95% CI: 1.24-20.92; p = 0.03).Conclusion Salivary MMP-8 and IL-1 beta discriminate periodontitis in T2DM.