High throughput single-cell detection of multiplex CRISPR-edited gene modifications
GENOME BIOLOGY(2020)
摘要
CRISPR-Cas9 gene editing has transformed our ability to rapidly interrogate the functional impact of somatic mutations in human cancers. Droplet-based technology enables the analysis of Cas9-introduced gene edits in thousands of single cells. Using this technology, we analyze Ba/F3 cells engineered to express single or multiplexed loss-of-function mutations recurrent in chronic lymphocytic leukemia. Our approach reliably quantifies mutational co-occurrences, zygosity status, and the occurrence of Cas9 edits at single-cell resolution.
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关键词
Genetics, Single cell, Genome editing, Loss-of-function, Mutation, CRISPR-Cas9, chronic lymphocytic leukemia
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