Multi-wavelength imaging of HPTLC plates using a constructed illumination chamber with a smartphone camera as the detector.

In this article, we present a new type of inexpensive multi-illumination source chamber. The innovation makes use of a smartphone camera which possesses the ability of capturing multiple images. Its performance was compared to a commercially available densitometer. Similar devices and suitable strategies for data analysis will help to solve diverse classification and/or regression problems, which will be far beyond a TLC characterization of ink samples. The multi-illumination chamber was used in an exemplary forensic application. The differences in the chemical composition of various brands of fountain pen inks were revealed on images of high-performance thin-layer chromatographic plates. Reducing image data simplified the visualization and facilitated a multivariate exploratory of the ink samples. Compared to the samples that were characterized by single wavelength densitograms, the multi-wavelength characterization using the illumination chamber with a smartphone camera or densitometer improved the clustering tendency of studied samples and enhanced their interpretation. The constructed chamber for multi-wavelength imaging is an inexpensive alternative (ca. 20 Euros) to the commercially available densitometers. The discussed approaches for image acquisition and chemometric data processing support a more reliable and objective analysis of TLC multi-wavelength data.