Antioxidant Compounds From Banisteriopsis Argyrophylla Leaves As Alpha-Amylase, Alpha-Glucosidase, Lipase, And Glycation Inhibitors

Banisteriopsis argyrophylla belongs to the Malpighiaceae family, which is a species from Cerrado, also known as "cip ' o-prata" or "cip ' o-folha-de-prata." Several species of this family present biological potential. This work reports the chemical identification of the ethanol extract (EE) and its fractions from B. argyrophylla leaves and shows the analysis of the antioxidant activity and inhibitory effects on activities of alpha-amylase, alpha-glucosidase and lipase, and non-enzymatic glycation. The ethyl acetate fraction (EAF) and n-butanol fraction (BF) showed antioxidant activity, with IC50 values of 4.1 +/- 0.1 and 4.8 +/- 0.1 mu g mL(-1), respectively, by the 2,2-diphenyl-1picrylhydrazyl (DPPH) method, and IC50 values of 6046.3 +/- 174.2 and 6264.2 +/- 32.2 mu mol Trolox eq g(-1) by the oxygen radical absorbance capacity (ORAC) method. Furthermore, the DPPH method with these fractions presented electroactive species with antioxidant potential, as shown by the differential pulse voltammetry (DPV) method. The inhibitory effects of the EAF and BF were demonstrated by the following results: IC50 of 5.1 +/- 0.3 and 2.5 +/- 0.2 mu g mL(-1) for alpha-amylase, IC50 of 1093.5 +/- 26.0 and 1250.8 +/- 21.9 mu g mL(-1) for alpha-glucosidase, IC50 of 8.3 +/- 4.1 and 4.4 +/- 1.0 mu g mL(-1) for lipase, and IC50 of 1.3 +/- 0.1 and 0.9 +/- 0.1 mu g mL(-1) for glycation. Some bioactive compounds were identified by (-)-ESI-MS/MS, such as catechin, procyanidins, glycosylated flavonoids, kaempferol, and megastigmane glucosides. The antidiabetic activity of B. argyrophylla has been reported for the first time.