Functional Evaluation Of Anti-Tnf-Alpha Affibody Molecules In Biochemical Detection And Inhibition To Signalling Pathways Of A Synovial Cell

Background: An affibody molecule obtained from a bioengineered staphylococcal protein was previously shown to act as an affinity binder for a wide range of targets and develop Tumour Necrosis Factor alpha (TNF-alpha)-binding clones.Methods: In this study, we demonstrated that affibody molecules against TNF-alpha could bind to recombinant TNF-alpha on the membrane for biochemical detection. In addition, we examined whether the affibody molecules could block binding between recombinant TNF-alpha and its receptor on MH7A synovial cells.Results: When a TNF-alpha-binding affibody was added, the production level of inflammatory mediators IL-6 and MMP-3 in MH7A were found to decrease up to 44%. Additionally, proliferation of synovial cells was also inhibited by the addition of TNF-alpha to cultivation media.Conclusion: These results suggest that affibody molecules against TNF-alpha could be candidate molecules for the detection of TNF-alpha during biochemical analysis and pharmacotherapy for rheumatoid arthritis.