Xylomelum Occidentale(Proteaceae) Accesses Relatively Mobile Soil Organic Phosphorus Without Releasing Carboxylates

Hundreds of Proteaceae species in Australia and South Africa typically grow on phosphorus (P)-impoverished soils, exhibiting a carboxylate-releasing P-mobilizing strategy. In the Southwest Australian Biodiversity Hotspot, twoXylomelum(Proteaceae) species are widely distributed, but restricted within that distribution. We grewXylomelum occidentalein hydroponics at 1 mu M P. Leaves, seeds, rhizosheath and bulk soil were collected in natural habitats. Xylomelum occidentaledid not produce functional cluster roots and occupied soils that are somewhat less P-impoverished than those in typical Proteaceae habitats in the region. Based on measurements of foliar manganese concentrations (a proxy for rhizosphere carboxylate concentrations) and P fractions in bulk and rhizosheath soil, we conclude thatX. occidentaleaccesses organic P, without releasing carboxylates. Solution(31)P-NMR spectroscopy revealed which organic P formsX. occidentaleaccessed. Xylomelum occidentaleuses a strategy that differs fundamentally from that typical in Proteaceae, accessing soil organic P without carboxylates. We surmise that this novel strategy is likely expressed also in co-occurring non-Proteaceae that lack a carboxylate-exuding strategy. These co-occurring species are unlikely to benefit from mycorrhizal associations, because plant-available soil P concentrations are too low. Synthesis. Our findings show the first field evidence of effectively utilizing soil organic P byX. occidentalewithout carboxylate exudation and explain their relatively restricted distribution in an old P-impoverished landscape, contributing to a better understanding of how diverse P-acquisition strategies coexist in a megadiverse ecosystem.