Low Frequency Vortex Magnetic Field Reduces Amyloid Beta Aggregation, Increase Cell Viability And Protect From Amyloid Beta Toxicity

Plaques formed by abnormal accumulation of amyloid beta-peptide (A beta) lead to onset of Alzheimer's disease (AD). Pharmacological treatments do not reduce A beta aggregation neither restore learning and memory. Noninvasive techniques have emerged as an alternative to treat AD, such as stimulation with electromagnetic fields (EMF) that decrease A beta deposition and reverses cognitive impairment in AD mice, even though some studies showed side effects on parallel magnetic fields stimulation. As a new approach of magnetic field (MF) stimulation, vortex magnetic fields (VMF) have been tested inducing a random movement of charged biomolecules in cells, promoting cell viability and apparently safer than parallel magnetic fields. In this study we demonstrate the effect of VMF on A beta aggregation. The experimental strategy includes, i) design and construction of a coil capable to induce VMF, ii) evaluation of VMF stimulation on A beta peptide induced-fibrils-formation, iii) evaluation of VMF stimulation on SH-SY5Y neuroblastoma cell line in the presence of A beta peptide. We demonstrated for the first time that A beta aggregation exposed to VMF during 24 h decreased similar to 86% of A beta fibril formation compared to control. Likewise, VMF stimulation reduced A beta fibrils-cytotoxicity and increase SH-SY5Y cell viability. These data establish the basis for future investigation that involve VMF as inhibitor of A beta-pathology and indicate the therapeutic potential of VMF for AD treatment.