Oxymatrine Inhibits The Development Of Non-Small Cell Lung Cancer Through Mir-367-3p Upregulation And Target Gene Sgk3 Downregulation

Oxymatrine (OM), an important active ingredient extracted from sophora flavescens, has attracted more attention for its anti-tumor effect in recent years, with pronounced effects on the development of multiple tumors, acting as a potential effective low toxic drug in clinical tumor treatment. In this study, CCK-8 and transwell experiments were applied to detect cell proliferation and migration. Quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting were used to test the expression of miR-367-3p and serum and glucocorticoid regulated kinase 3 (SGK3). The function of oxymatrine in non-small cell lung cancer (NSCLC) progression was also confirmed in vivo. Then, CCK-8 and transwell assays revealed that oxymatrine could repress NSCLC cell migration and proliferation. qRT-PCR showed the striking promotion roles of oxymatrine in cancer suppressor gene miR-367-3p expression. The results of further dual luciferase reporter gene experiment demonstrated that SGK3 was a target gene of miR-367-3p and under the regulation of oxymatrine. The rescue experiments indicated that OM functioned via miR-367-3p, while miR-367-3p exerted its function by action on SGK3. Finally, in vivo studies showed that OM could also inhibit tumor growth. As a result, this study found that OM inhibited the development of NSCLC through reducing the expression of a downstream target gene SGK3 by promoting miR-367-3p expression.