Inhibitory Effect Of Imperatorin On The Pharmacokinetics Of Diazepam In Vitro And In Vivo

Background Diazepam is a benzodiazepine drug used to treat anxiety, insomnia, and muscle spasms. Imperatorin is a phytochemical isolated from medicinal plants and is widely used in herbal medicine. The aim of this study was to investigate the interactions between imperatorin and diazepamin vitroandin vivoand to provide evidence-based guidance for the safe clinical use of the drug. Methods In vitro inhibition of imperatorin was assessed by incubating rat liver microsomes with diazepam to determine IC(50)values and the type of inhibition. Forin vivoassessment, six rats were pretreated with 50 mg/kg imperatorin for two weeks, six were administered saline, and a single dose of 10 mg/kg diazepam was administered orally to both groups 30 min after the administration of imperatorin. Results Imperatorin inhibited thein vitrometabolism of diazepamviathe competitive mechanism of CYP450. The IC(50)values of imperatorin to nordazepam and temazepam were 1.54 mu M and 1.80 mu M, respectively. The inhibitory constant values for temazepam and nordazepam were 1.24 mu M and 1.29 mu M, respectively. Long-term administration of imperatorin significantly increased the AUC((0-12h)), AUC((0-infinity)), and Cmax of diazepam, while Vz/F and CLz/F were decreased significantly (P< 0.05). In turn, the AUC((0-12h)), AUC((0-infinity)), and Cmax of nordazepam and temazepam decreased significantly, and Vz/F and CLz/F increased significantly (P< 0.05). Conclusions This study demonstrates that imperatorin inhibits the metabolism of diazepam bothin vitroandin vivo. These results indicated that more attention should be paid when taking diazepam together with food or herbs containing IMP, although further investigation is still needed.