Efficient ex situ biosynthesis of vitamin B12 by Propionibacterium freudenreichii using membrane separation coupling technology

Ziqiang Wang,Guoxia Xu,Wei Du, Yuhan Zhang,Yunshan Wang,Zhiguo Su

Biochemical Engineering Journal（2020）

引用 4|浏览6

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摘要

•Ado-cbi was used as the indicator signal to determine the addition time of DMB.•Membrane separation technology was applied to vitamin B12 ex-situ biosynthesis.•The semi-continuous fermentation of P. freudenreichii was designed.

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关键词

Propionibacterium freudenreichii,Vitamin B12,Adenosylcobinamide,Ex-situ biosynthesis,Membrane separation coupling fermentation

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