Research on Regulation of Receptor Signalling Pathway of HeLa Cells by Clock Molecule DEC1

Objective: To make a study of the key factors from the Src to the nuclear transcription factor ERK signaling pathway as the target and to explore the role of DEC1 mediated ERK signaling pathway in cervical cancer and its mechanism. Methods: On the basis of screening DEC1 positive cells, the Hela cells with the highest positive rate of FRtt and the lowest DEC1 positive rate of MCF-7 cells were used as the experimental objects, and the growth of cervical cancer cells was detected by cell counting. Results: Src ERKl and C Hela cells, Jun mRNA relative expression was significantly lower than that in MCF-7 cells, the differences were statistically significant (P< 0.05): Hela and MCF-7 cells ERK2 and C -Fos mRNA relative expression was no significant difference (P> 0.05). Conclusion: DEC1 can down regulate the expression of ERK upstream factor Src and protein in Hela cells of cervical cancer cell line mRNA, suggesting that DEC1 has other intermediate factors in the activation of ERK signaling pathway.