Comparison and evaluation of two pretreatments of different mechanisms for multi-Fusarium toxins

A simultaneous determination method for four kinds of Fusarium toxins (zearalenone, deoxynivalenol, T-2 and HT-2 toxins) in flour was established using high performance liquid chromatography-tendem mass spectrometry (LC-MS/MS). Samples were cleaned up with MycoSep (R) 226 multifunction purifying columns and immunoaffinity columns DZT MS-PREP separately. The recovery of the Mycosep (R) 226 multifunction purifying column is 69.42%similar to 111.12%, and the relative standard deviation is 1.46%similar to 13.24%. The recovery of the immunoaffinity columns DZT MS-PREP is 88.01%similar to 107.31%, and the relative standard deviation is 1.09%similar to 14.42%. 7 kinds of flour samples were determined, most of the detection results of the immunoaffinity columns DZT MS-PREP were higher than that of the Mycosep (R) 226 multifunction purifying column. The immunoaffinity columns provides an alternative effective and fast tool for simultaneous extraction of the residues of four Fusariumtoxins from real samples.