Mechanisms of low cadmium accumulation in storage root of sweetpotato (Ipomoea batatas L.)

Sweetpotato (Ipomoea batatas L.) possess great application prospects due to their low cadmium (Cd) concentration within their storage roots despite growth on Cd-polluted fields. The mechanisms of low Cd accumulation in storage root is not entirely clear. We found that the blocking effect of Cd uptake in the root absorption system and the characteristics of Cd distribution in storage root play a decisive role in the regulation of low Cd accumulation in storage root. Cd absorbed from the rhizosphere mainly accumulated in feeder roots in Cd dose-dependent accumulation analyses. Meanwhile, we found that Cd absorbed by the peels of storage root was mainly transported from peels to shoots, rather than directly into the fleshed storage root. Further analysis indicated that Cd uptake, transport, and distribution in sweetpotato hinges on whether Cd enters the plant plasma membrane by either the symplast or apoplast pathway. The Cd concentration in feeder root decreased after respiratory inhibitors CCCP and DNP treatment and increased after the culture temperature was raised from 28 ℃ to 35 ℃. Non-invasive microelectrode Cd flux measurements further revealed that Cd uptake in feeder root was affected greatly by the Cd concentration of the solution and was markedly reduced by respiratory inhibitor CCCP. Relative to the elongation zone and mature zone, the meristematic zone was the main site of Cd uptake in the root absorption system. This study suggests that inhibition of Cd uptake by the root absorption system and the characteristics of Cd distribution in storage root are the main reasons for low cadmium accumulation in storage root.