Fine Mapping Of The Ph-2 Gene Conferring Resistance To Late Blight (Phytophthora Infestans) In Tomato
PLANT DISEASE(2021)
摘要
Late blight is a devastating tomato disease. Breeding new varieties with multiple resistance (R) genes is highly effective for preventing late blight. The Ph-2 gene mediates resistance to Phytophthora infestans race T-1 in tomato. In this study, we used an F-2 population derived from a cross between Solanum lycopersicum Moboline (resistant) and LA3988 (susceptible) cultivars for the fine mapping of Ph-2. Two flanking markers, CAPS-1 and CC-Ase, mapped Ph-2 to a 141-kb genomic region containing 21 projected genes, 5 of which were identified as putative R genes. The Solyc10g085460 coding sequence varied significantly between the parents. The markers developed and candidate genes identified in this study shall be useful for the molecular breeding of tomato exhibiting increased late blight resistance and for the cloning of the Ph-2 gene.
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关键词
candidate genes, fine mapping, late blight, Ph-2, tomato
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