Preliminary Study on a RT-PCR Method for the Equine Infectious Anemia Virus Detection

To develop a Reverse Transcription-Polymerase Chain Reaction (RT-PCR) method for quick detecting equine infectious anemia virus (EIAV), according the conserved sequences of EIAV gag gene which published in GenBank, 2 specific RT-PCR primers and 8 extension primers were designed and synthesized. A DNA fragment of EIAV gag gene about 370bp was synthesized in vitro by overlapping PCR, then the RT-PCR method for equine infectious anemia detection was established preliminarily through optimizing the reaction conditions, sensitivity and specificity experiments, and validity test. The results showed that the method for the EIAV has a good specificity and sensitivity, and the reaction product was about 370bp DNA electro phoretic band. This method can detect the EIAV target fragment about 119copies/mu L. There are no amplification for the detection of Proteus, Actinobacillus, Salmonella, Escherichia coli, Stenotrophomonasmalt ophilia and Streptococcus type II, and the results showed positive reaction for Equine infectious anemia test antigen detection.