Transcriptional Regulation Ofprolactinin A Euryhaline Teleost: Characterisation Of Gene Promoters Through In Silico And Transcriptome Analyses

The sensitivity of prolactin (Prl) cells of the Mozambique tilapia (Oreochromis mossambicus) pituitary to variations in extracellular osmolality enables investigations into how osmoreception underlies patterns of hormone secretion. Through the actions of their main secretory products, Prl cells play a key role in supporting hydromineral balance of fishes by controlling the major osmoregulatory organs (ie, gill, intestine and kidney). The release of Prl from isolated cells of the rostral pars distalis (RPD) occurs in direct response to physiologically relevant reductions in extracellular osmolality. Although the particular signal transduction pathways that link osmotic conditions to Prl secretion have been identified, the processes that underlie hyposmotic induction ofprlgene expression remain unknown. In this short review, we describe two distinct tilapia gene loci that encode Prl(177)and Prl(188). From our in silico analyses ofprl(177)andprl(188)promoter regions (approximately 1000 bp) and a transcriptome analysis of RPDs from fresh water (FW)- and seawater (SW)-acclimated tilapia, we propose a working model for how multiple transcription factors link osmoreceptive processes with adaptive patterns ofprl(177)andprl(188)gene expression. We confirmed via RNA-sequencing and a quantitative polymerase chain reaction that multiple transcription factors emerging as predicted regulators ofprlgene expression are expressed in the RPD of tilapia. In particular, gene transcripts encodingpou1f1,stat3,creb3l1, pbxip1aandstat1awere highly expressed;creb3l1, pbxip1aandstat1awere elevated in fish acclimated to SW vs FW. Combined, our in silico and transcriptome analyses set a path for resolving how adaptive patterns of Prl secretion are achieved via the integration of osmoreceptive processes with the control ofprlgene transcription.