Primer pair for identifying newcastle disease virus and multi subtype avian influenza virus and application thereof

The invention discloses a primer pair for identifying newcastle disease virus and multi-subtype avian influenza virus and an application thereof. The PCR primer pair composition provided by the invention comprises primer pair NDV, primer pair H3V, primer pair H5V, and primer pair H9V; the PCR primer pair composition is applicable to the preparation of reagents for the diagnosis or auxiliary diagnosis of newcastle disease and avian influenza, or to the preparation of reagents for the identification or auxiliary identification of newcastle disease virus and multi-subtype avian influenza virus. When the PCR primer pair composition provided by the invention is used to simultaneously detect newcastle disease virus, H3, H5 and H9-subtype avian influenza virus, the specificity is strong; the sensitivity is 1EID50/100 microliters, 1EID50/100 microliters, 1*10-2EID50/100 microliters, and 1EID50/100 microliters respectively; compared with identification results of routine test methods such as virus isolation and hemagglutination inhibition tests, the results obtained by using the PCR primer pair composition of the invention has a coincidence rate of up to 100%; the PCR primer pair composition provided by the invention is applicable to the development of corresponding multiplex RT-PCR kits for the clinical diagnosis and epidemiological control of newcastle disease and avian influenza.