Leeia Aquatica Sp. Nov., Isolated From Freshwater

A Gram stainnegative, rodshaped, obligately aerobic, motile by a single polar flagellum, chemoheterotrophic bacterium, designated strain IMCC25680(T), was isolated from surface water in Chungju Lake, Republic of Korea. 16S rRNA gene sequence analysis revealed that strain IMCC25680(T) was most closely related to Leeia oryzae HW7(T) with 95.5% sequence similarity and formed a robust clade with L. oryzae HW7(T). Whole genome sequencing showed that strain IMCC25680(T) had a genome 3.6 Mbp long with 60.7 mol% DNA G+C content. Average nucleotide identity and digital DNA-DNA hybridization values between strain IMCC25680(T) and L. oryzae HW7(T) were 72.4% and 18.5%, respectively, indicating that the novel strain represents a novel species of the genus Leeia. The major cellular fatty acids of strain IMCC25680(T) were iso-C-16:0 and summed feature 3 (comprising C-16:1 omega 6c and/or C-16:1 omega 7c). The respiratory quinone detected in the strain was ubiquinone-8. The major polar lipids were found to be phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and two unidentified polar lipids. On the basis of the phylogenetic and phenotypic characterization, strain IMCC25680(T) was considered to represent a novel species within the genus Leeia, for which the name Leeia aquatica sp. nov. is proposed. The type strain is IMCC25680(T) (=KACC 19487(T) =NBRC 113132(T)).