Identification And Characterization Of The Long Noncoding Rna Dreg1 As A Novel Regulator Of Gata3

The eukaryotic genome is three-dimensionally segregated into discrete globules of topologically associating domains (TADs), within which numerouscis-regulatory elements such as enhancers and promoters interact to regulate gene expression. In this study, we identify a T-cell-specific sub-TAD containing theGata3locus, and reveal a previously uncharacterized long noncoding RNA (Dreg1) within a distant enhancer lying approximately 280 kb downstream ofGata3. Dreg1 expression is highly correlated with that of Gata3 during early immune system development and T helper type 2 cell differentiation. Inhibition and overexpression ofDreg1suggest that it may be involved in the establishment, but not in the maintenance ofGata3expression. Overall, we propose thatDreg1is a novel regulator ofGata3and may inform therapeutic strategies in diseases such allergy and lymphoma, whereGata3has a pathological role.