Genome assembly and annotation of Macadamia tetraphylla

Macadamia is a kind of evergreen nut trees which belong to the Proteaceae family. The two commercial macadamia species, and , are highly prized for their edible kernels. Catherine et al. reported genome using NGS sequencing technology. However, the lack of a high-quality assembly for hinders the progress in biological research and breeding program. In this study, we report a high-quality genome sequence of using the Oxford Nanopore Technologies (ONT) technology. We generated an assembly of 750.54 Mb with a contig N50 length of 1.18 Mb, which is close to the size estimated by flow cytometry and k-mer analysis. Repetitive sequence represent 58.57% of the genome sequence, which is strikingly higher compared with . A total of 31,571 protein-coding genes were annotated with an average length of 6,055 bp, of which 92.59% were functionally annotated. The genome sequence of will provide novel insights into the breeding of novel strains and genetic improvement of agronomic traits.