The secreted protein signature of hydatid fluid from pulmonary cystic echinococcosis

The vast majority of cystic echinococcosis cases in Southern Brazil are caused by and . Comparative proteomic studies of helminths have increased the knowledge about the molecular survival strategies adopted by parasites. Here, we surveyed the protein contents of the hydatid fluid compartment of and pulmonary bovine cysts, in an attempt to compare their molecular arsenal in this host-parasite interface. Hydatid fluid samples from three isolates of each species were analyzed by trypsin digestion and mass spectrometry. We identified 280 proteins in and 251 proteins in , highlighting a core of 52 proteins common to all samples of hydatid fluid. The functional analysis revealed important molecular functions and processes active in pulmonary cystic echinococcosis. Some were more evident in one species, such as apoptosis in , and cysteine protease activity in , while many molecular activities have been found in fluids of both species, such as proteolysis, development signaling and extracellular structures organization. The similar molecular tools employed by and for their survival within the host are potential targets for new therapeutic approaches to deal with cystic echinococcosis and other larval cestodiases.