The Effect of Introgression of an R2R3 MYB Transcription Factor on Sulphur Metabolism in Brassica oleracea
biorxiv(2020)
摘要
Background A diet rich in cruciferous vegetables is reported to have beneficial health effects, partially mediated by 4-methylsulfinylbutyl glucosinolate, or glucoraphanin, which is predominantly found within broccoli ( Brassica oleracea var italica ). We describe the downstream effects on transcription and metabolism in broccoli following the introgression of a genetic variant of MYB28 into broccoli from a wild Brassica relative which has previously been associated with enhancement of glucoraphanin.
Results Whole genome sequencing, RNA expression and metabolite analyses were used to characterise the consequences of the introgression of either one or two copies of a genetic variant of the MYB28 transcription factor into a commercial broccoli genetic background. The introgression of the variant of MYB28 resulted in enhanced expression of genes involved in primary sulphate assimilation, sulphur metabolism and aliphatic glucosinolate biosynthesis, and enhanced accumulation of 4-methylsulphinyl butyl glucosinolate in florets. Other changes in transcription that may be related to non-targeted introgression events are reported. There were no consistent effects upon sulphur metabolites pools, apart from methionine-derived glucosinolates.
Conclusion This study illustrates the downstream effects on transcription and metabolism of the introgression of a genetic variant of MYB28 from a wild species into a commercial broccoli genotype.
### Competing Interest Statement
The authors have declared no competing interest.
* 3-MSP
: 3-methylsulfinylpentyl
4-MSB
: 4-methylsulfinylbutyl
ANOVA
: Analysis of Variance
GO
: Gene Ontology
HAG1
: High Aliphatic Glucosinolate 1
HP-LC
: High Performance Liquid Chromatography
I3M
: Indolyl methyl
1MOI3M
: 1-methoxyindolyl methyl
4MOI3M
: 4-methoxyindolyl methyl
MDS
: Multidimensional Scaling
FPKMs
: Fragments Per Kilobase Million
SMCSO
: S-methyl cysteine sulfoxide
TPM
: Transcripts Per Million
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关键词
Broccoli,<italic>Brassica oleracea</italic>,Glucoraphanin,Transcription Factor,Transcriptome,Sulphur Metabolism
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