Cloning and In silico characterization of Heat shock factor (Hsf2) from Wheat ( Triticum aestivum L. )

A gene encoding putative Heat shock Factor (Hsf) from Wheat (Triticum aestivum L.) was characterized and designated as TaHsf (Gen Bank ID: JQ801451) while the genomic sequence of Hsf was found to be 1497 bp long with the open reading frame of 1299 bp in length. The deduced amino acid sequence of CA contained HSF type conserved domains, which showed high degree of homology with other hsf genes of related species. We have reported three-dimensional model of TaHsf and quality of the predicted model analysed with PROCHECK. The expression of TaHsf transcripts was studied in different tissues and different developmental stages. The HSFs being the central mediator for the expression of HSPs play very important role in mediating tolerance to abiotic stress especially heat stress. The sudden increase in temperature year by year has adversely affected the growth and productivity of winter wheat (Triticum aestivum L.). In wheat, a limited number of HSfs are being reported and investigated, thus calling for the characterization of more HSP's coding HSFs. In our study we have cloned one of the novel HSFs and have characterized it through in silico tools.