Cell-Type Specificity of Neuronal Excitability and Morphology in the Central Amygdala
ENEURO(2021)
Abstract
Central amygdala (CeA) neurons expressing protein kinase C delta (PKC delta(+)) or somatostatin (Som(+)) differentially modulate diverse behaviors. The underlying features supporting cell-type-specific function in the CeA, however, remain unknown. Using whole-cell patch-clamp electrophysiology in acute mouse brain slices and biocytin-based neuronal reconstructions, we demonstrate that neuronal morphology and relative excitability are two distinguishing features between Som(+) and PKC delta(+) neurons in the laterocapsular subdivision of the CeA (CeLC). Som(+) neurons, for example, are more excitable, compact, and with more complex dendritic arborizations than PKC delta(+) neurons. Cell size, intrinsic membrane properties, and anatomic localization were further shown to correlate with cell-type-specific differences in excitability. Lastly, in the context of neuropathic pain, we show a shift in the excitability equilibrium between PKC delta(+) and Som(+) neurons, suggesting that imbalances in the relative output of these cells underlie maladaptive changes in behaviors. Together, our results identify fundamentally important distinguishing features of PKC delta(+) and Som(+) cells that support cell-type-specific function in the CeA.
MoreTranslated text
Key words
central amygdala,intrinsic excitability,morphology,neuropathic pain,protein kinase C delta,somatostatin
AI Read Science
Must-Reading Tree
Example
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
Generate MRT to find the research sequence of this paper
Chat Paper
Summary is being generated by the instructions you defined